目的 对女贞子有效成分特女贞苷的含量和抗氧化活性随时间和温度变化规律进行研究，确定女贞子适宜贮藏期。 方法 采用高效液相法对经典恒温法加速试验前后的女贞子中特女贞苷进行含量测定，获得特女贞苷的动力参数，根据Arrhenius指数定律预测女贞子在室温下的有效期。采用2，2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)体外抗氧化活性分析方法，对女贞子加速试验前后进行抗氧化活性研究。 结果 女贞子在25 ℃下的有效期大约为1.83年。经加速试验后女贞子抗氧活化性呈先升高后下降趋势。 结论 贮藏时间和温度会影响女贞子药材的化学成分，贮存时应在阴凉处保存，存放时间不超过1.83年。
Abstract
OBJECTIVE To study the change rule of specnuezhenide content and antoxidant activity of Ligustri Lucidi Fructus with temperature and time, and determine its validity period. METHODS Dynamic parameters were established under constant temperature, and specnuezhenide content of Ligustri Lucidi Fructus was determined by HPLC after accelerated test by classical constant temperature method. The shelf life of Ligustri Lucidi Fructus at 25 ℃ were determined by Arrhenius theory. The antioxidant activities of different samples of Ligustri Lucidi Fructus were evaluated by ABTS assay. RESULTS The shelf life of Ligustri Lucidi Fructus was forecasted to be 1.83 years at 25 ℃. The antioxidant activitiy of Ligustri Lucidi Fructus showed a trend of decline after rising first. CONCLUSION High temperature and storage time would affect the chemical composition of Ligustri Lucidi Fructus, so Ligustri Lucidi Fructus should be kept in the shade for no more than 1.83 years for a good therapeutic effect.
关键词
女贞子 /
经典恒温法 /
特女贞苷 /
高效液相色谱法 /
抗氧化
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Key words
Ligustri Lucidi Fructus /
classical constant temperature method /
specnuezhenide /
HPLC /
antoxidant activity
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中图分类号：
R282
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脚注
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基金
河南省高校科技创新团队(16IRTSTHN019);2015年河南省教育技术装备和实践教育研究立项课题(GZS008);河南省教育厅科学技术研究重点项目(16A360008);开封市创新人才项目(1509010)
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